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PCR was performed using a 2720 thermal cycler (Applied Biosystems, Foster City, CA, USA) programmed for 2 min at 98C for initial denaturation, followed by 30 cycles of 10 s at 98C, 30 s at 55C, 30 s at 72C, and a final extension of 1 min at 72C

Richter, K

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were both full professors, she says

The tobacco and tobacco alternatives industry is a dynamic space, constantly shifting with new technologies, regulations, and market demands

In addition, the widespread availability of smartphones96% of US adults 1829 years old owned one as of 2022has helped extend the reach of smoking cessation apps to a large number of young adults who may not otherwise have access to cessation services [25]